Total Phenolic Content Folin Ciocalteu Method Principle

This study presents a review of the folin ciocalteu f c assay for total phenolic content tpc determinations and describes different approaches to improve its specificity.

Total phenolic content folin ciocalteu method principle. Phenolic quantification assay is based on folin ciocalteu method. A single laboratory validation of a method using folin ciocalteu s phenol reagent folin c reagent for determination of total phenolic content of selected dietary supplement extracts was performed. These solutions will have phenol concentrations of 0 50 100 150 250 and 500 mg l gallic acid the effective range of the assay. Briefly 200 μl of crude extract 1 mg ml were made up to 3 ml with distilled water mixed thoroughly with 0 5 ml of folin ciocalteu reagent for 3 min followed by the addition of 2 ml of 20 w v sodium carbonate.

The method is composed of a water extraction of dried extracts with sonication followed by reaction with the folin c reagent. The method relies on the transfer of electrons in alkaline medium from phenolic compounds to form a blue chromophore constituted by a phosphotungstic phosphomolybdenum complex where the maximum. Left over gallic acid solutions can be poured down the drain. The folin ciocalteu f c reaction is an antioxidant assay based on electron transfer which measures the reductive capacity of an antioxidant.

The total phenolic content of the extract was determined by the folin ciocalteu method.